tamalodge.blogg.se - Ring map civ 5

Amino-acid changes to the atomic model (with respect to the mt-DNA sequence) were only made for amino-acid positions that had unambiguous cryoEM density evidence and whose editing is conserved or would restore the conserved sequence. The existence of the RNA-editing site in other plants, or the implied restoration of the consensus sequence was then inspected. RNA-editing sites were initially identified by an unambiguous mismatch in the cryoEM density and the expected density for the mitochondrial-DNA-encoded residue. Additional BLASTp searches were performed as needed. Mammalian and yeast homologs were obtained from Hartley et al., 2019 Maréchal et al., 2012. radiata homologs were obtained by performing BLASTp searches of the Arabidopsis thaliana genes ( Meyer et al., 2019). ( c) CIII 2 and CIV subunit homologs in plants, yeast and mammals. ( b) Model-building statistics by subunit. Supplementary file 1: ( a) Mass spectrometry identification of V.

Electron Microscopy Public Image Archive. radiata mitochondrial membranes - mixed fraction containing CI*, CIII2 and SC III2+IV. Cryo electron micrographs of digitonin-solubilized, amphipol-stabilized, sucrose-gradient-purified V.

Maldonado M, Padavannil A, Zhou L, Guo F, Letts JA.

Plant mitochondrial complex III2 from Vigna radiata supercomplex III2+IV, focused refinement. Plant mitochondrial supercomplex III2+IV from Vigna radiata. Plant Mitochondrial complex SC III2+IV from Vigna radiata composite map. Plant Mitochondrial complex IV from Vigna radiata. Plant Mitochondrial complex III2 from Vigna radiata. Plant Mitochondrial complex SC III2+IV from Vigna radiata. Mass spectrometry data for Vigna radiata concentrated, pooled peak 2 fractions from sucrose gradient, Maldonado et al 2020.